Tell us about yourself!

Complete Your Profile
  • Becoming With Schizophyllum Commune: Create a Sterile Culture From Found Mushrooms

    Wow!Thank you so much! That document is amazing! I've got great news, a collaborator of the Natural History Museum found and identified the mushrooms, so we are confident with them. They are already growing in agar plates, some seeded with the tissue and some with the spores, and we are waiting for the mycelium to grow. The tissue was super dry yesterday, but after ~20h in agar it had sponged and the gills were open. It was looking quite happy. The Remazol Brilliant Blue is not available on time, so we'll be using methyl blue as an indicator of the "digesting" activity of the fungi. It feels a bit like a test run more that the experiment, but I guess it'll be alright. The project on the PCR machine is also advancing and I am just floating that I'll be able to join the two!Frid...

    see more »

    Wow!Thank you so much! That document is amazing! I've got great news, a collaborator of the Natural History Museum found and identified the mushrooms, so we are confident with them. They are already growing in agar plates, some seeded with the tissue and some with the spores, and we are waiting for the mycelium to grow. The tissue was super dry yesterday, but after ~20h in agar it had sponged and the gills were open. It was looking quite happy. The Remazol Brilliant Blue is not available on time, so we'll be using methyl blue as an indicator of the "digesting" activity of the fungi. It feels a bit like a test run more that the experiment, but I guess it'll be alright. The project on the PCR machine is also advancing and I am just floating that I'll be able to join the two!Friday is my deadline to present the reports on the projects, but for the mushrooms I have a little extension on the deadline until the 18th March. Idk, would you like to have the documents? Maybe I'll just leave it laying around...PCRhttps://docs.google.com/document/d/1UPX9It7d1qcxSa...S. Communehttps://docs.google.com/document/d/1P_Yz1RBy3R4aVV...I have to thank you a lot for all your help. I wouldn't have found this research on my own and I have enjoyed a lot the project.Thanks, thanks thanks!Thanks againAmaya

    View Instructable »
  • Becoming With Schizophyllum Commune: Create a Sterile Culture From Found Mushrooms

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project ... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project :) ... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    View Instructable »
  • Arduino PCR (thermal Cycler) for Under $85

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    View Instructable »
  • Becoming With Schizophyllum Commune: Create a Sterile Culture From Found Mushrooms

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    Hey,It's been a while!I'm having a bit of trouble with the project because in here it is so cold! I cannot find anything alive! We found some fungi on rotting wood and collected them, but they are hard as chips, and I am not confident they will go back to life. However it would be great to identify them (or just know if I should throw them away). We are trying to develop some project for our science fair, and we are making this DIY PCR https://www.instructables.com/id/Arduino-PCR-therm...I was going to ask you about the genetic identification part of your project �... Just to know if you could give us a hint of what to do once we get finished our PCR machineI attach the pictures of what we have found so far, Thanks a lot! and best regards, Amaya and June-Si (partners on the project)

    View Instructable »
  • Arduino PCR (thermal Cycler) for Under $85

    Hey!I am doing this PCR as a part of a science fair competition project! I have a doubt on the depth of the cut in this part of the aluminium block... Is it possible that it is one centimetre and the label in the picture is not placed on the right spot? (it is the red bit on the pic)Also, the Arduino board we have is not the mini, it is the UNO. I guess we'll have to make a case with other dimensions? Thanks a lot for the Instructable! Amaya

    View Instructable »
  • Becoming With Schizophyllum Commune: Create a Sterile Culture From Found Mushrooms

    Good morning!Thanks a lot for your answer!The assays I found are called YES/YAS assays (for Yeast Estrogen or Androgen Screen) and they are produced by Xenometrix. http://www.xenometrix.ch/en/products/details/endoc...In their websit I cannot see the prices but their official seller in UK (Cambio) sells one kit for like 285 pounds + a yeast strain vial for around 200.(https://www.cambio.co.uk/0/1487/132/products/xenos... That goes way out of my budget!I think I'll go for the dye. Do you think they will sell it to a school laboratory? I can only think of my Bio teacher to place the order for me... Also, I wanted to ask for about how long does it take for the fungi to eat the dye, and if you think it is possible to control it with a spectrometer in order to get some obective measurement of...

    see more »

    Good morning!Thanks a lot for your answer!The assays I found are called YES/YAS assays (for Yeast Estrogen or Androgen Screen) and they are produced by Xenometrix. http://www.xenometrix.ch/en/products/details/endoc...In their websit I cannot see the prices but their official seller in UK (Cambio) sells one kit for like 285 pounds + a yeast strain vial for around 200.(https://www.cambio.co.uk/0/1487/132/products/xenos... That goes way out of my budget!I think I'll go for the dye. Do you think they will sell it to a school laboratory? I can only think of my Bio teacher to place the order for me... Also, I wanted to ask for about how long does it take for the fungi to eat the dye, and if you think it is possible to control it with a spectrometer in order to get some obective measurement of the change. My school lab has some basic Pasco spectrometers and I think I could borrow them. Also, did you test the whole hiphae system or did you isolate the enzymes? Some of the studies I've seen have done it in different ways. Thanks again, amaya

    View Instructable »
  • Becoming With Schizophyllum Commune: Create a Sterile Culture From Found Mushrooms

    Hey!It's me again! I'm sorry to be insistent. I've done a little bit of research today looking into the "testing whether the fungi does something to the plastic". I've found a couple of test kits but they are quite expensive and I'm not sure of what I need... I also need a good research question on this, like an aim or some application...Idk, if you have a suggestion I'll be glad to take it!

    View Instructable »
  • AmayaSE followed Embroidery, Reuse, Rockets, Toys and 20 others channel 1 year ago